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- Title
DelGEF, a homologue of the Ran guanine nucleotide exchange factor RanGEF, binds to the exocyst component Sec5 and modulates secretion
- Authors
Sjölinder, Mikael; Uhlmann, Jörg; Ponstingl, Herwig
- Abstract
In order to identify the function of deafness locus putative guanine nucleotide exchange factor (DelGEF), a protein homologous to the nucleotide exchange factor for the small GTPase Ran, a cDNA library was screened for interacting proteins using a yeast two-hybrid system. The human homologue of Sec5, a protein involved in vesicle transport and secretion, was identified as a binding partner. The interaction between DelGEF and Sec5 was found to be dependent on Mg2+ and stimulated by guanosine triphosphate (GTP) or deoxycytidine triphosphate (dCTP). Downregulation of endogenous DelGEF in HeLa cells induced increased extracellular secretion of proteoglycans indicating a possible role for DelGEF in the secretion process.
- Subjects
HELA cells; GUANOSINE triphosphatase
- Publication
FEBS Letters, 2002, Vol 532, Issue 1/2, p211
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)03677-3