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- Title
Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase †.
- Authors
Ahmad, Norlaily; Colak, Burcu; Zhang, De-Wen; Gibbs, Martin John; Watkinson, Michael; Becer, C. Remzi; Gautrot, Julien E.; Krause, Steffi
- Abstract
Peptide cross-linked poly(ethylene glycol) hydrogel has been widely used for drug delivery and tissue engineering. However, the use of this material as a biosensor for the detection of collagenase has not been explored. Proteases play a key role in the pathology of diseases such as rheumatoid arthritis and osteoarthritis. The detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a protease biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film and demonstrated for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals, and their degradation in the presence of collagenase was monitored using a quartz crystal microbalance (QCM). The biosensor was shown to respond to concentrations between 2 and 2000 nM in less than 10 min with a lower detection limit of 2 nM.
- Subjects
POLYETHYLENE glycol; HYDROGELS; BIOSENSORS; SURFACE coatings; COLLAGENASES; DRUG delivery devices
- Publication
Sensors (14248220), 2019, Vol 19, Issue 7, p1677
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s19071677