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- Title
Adipocyte-Derived Serum Amyloid A3 and Hyaluronan Play a Role in Monocyte Recruitment and Adhesion.
- Authors
Han, Chang Yeop; Subramanian, Savitha; Chan, Christina K.; Omer, Mohamed; Chiba, Tsuyoshi; Wight, Thomas N.; Chait, Alan
- Abstract
Obesity is characterized by adipocyte hypertrophy and macrophage accumulation in adipose tissue. Monocyte chemoattractant protein-1 (MCP-1) plays a role in macrophage recruitment into adipose tissue. However, other adipocyte-derived factors, e.g., hyaluronan and serum amyloid A (SAA), can facilitate monocyte adhesion and chemotaxis, respectively. The objective was to test the potential involvement of these factors in macrophage recruitment. Differentiated 3T3-L1 adipocytes made hypertrophic by growth in high glucose conditions were used to study SAA and hyaluronan regulation in vitro. Two mouse models of obesity were used to study their expression in vivo. Nuclear factor-κB was upregulated and peroxisome proliferator-activated receptor (PPAR)γ was downregulated in hypertrophic 3T3-L1 cells, with increased expression of SAA3 and increased hyaluronan production. Rosiglitazone, a PPARγ agonist, reversed these changes. Hypertrophic adipocytes demonstrated over-expression of SAA3 and hyaluronan synthase 2 in vitro and in vivo in diet-induced and genetic obesity. SAA and hyaluronan existed as part of a complex matrix that increased the adhesion and retention of monocytes. This complex, purified by binding to a biotinylated hyaluronan binding protein affinity column, also showed monocyte chemotactic activity, which was dependent on the presence of SAA3 and hyaluronan but independent of MCP-1. We hypothesize that adipocyte hypertrophy leads to increased production of SAA and hyaluronan, which act in concert to recruit and retain monocytes, thereby leading to local inflammation in adipose tissue. Diabetes 56:2260-2273, 2007
- Subjects
AMYLOID; FAT cells; MONOCYTES; HYALURONIC acid; OBESITY; ADIPOSE tissues; HYPERTROPHY; LABORATORY mice
- Publication
Diabetes, 2007, Vol 56, Issue 9, p2260
- ISSN
0012-1797
- Publication type
Article
- DOI
10.2337/db07-0218