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- Title
The MTHFR C677T and eNOS ID Polymorphisms Increase the Risk for ESRD in Romanian Diabetic and Nondiabetic Patients.
- Authors
Cimponeriu, DĂanut G.; Vlădică, Maria; Apostol, Pompilia P.; Panaite, Cristian; CrĂciun, Anne-Marie; Ungureanu, Dan; Moldovan, Cosmin; Serafinceanu, Cristian; Gavrilă, Lucian; Cheta, Dan M.
- Abstract
Diabetic patients have a high risk of developing end stage renal disease (ESRD). A large number of evidence suggests that this complication may involve genetic predisposition. Methylentetrahydrofolate reductase (MTHFR), angiotensin I-converting enzyme gene (ACE), endothelial nitric oxide synthase (eNOS) polymorphisms have been found to increase the risk for ESRD in some populations. The aim of this case-control study was to estimate the possible impact of MTHFR C677T, ACE-ID, eNOS 4a/5b polymorphisms on the ESRD development in diabetic and nondiabetic Romanian patients. Clinical information and blood samples were collected from 500 unrelated Caucasians. They were allotted into three groups: dDM (DM dialyzed patients, n=100, M:F=50:50), dnD (nondiabetic dialyzed patients, n=100, M:F =53:47) and HC (healthy controls, n=300, M:F=158:142). Genomic DNA was extracted from blood samples with Wizard Genomic DNA purification Kit (Promega). The ACE ID and eNOS ID polymorphisms was analyzed by PCR and the MTHFR C677T genotypes were established by PCR RFLP. The frequencies for these genotypes are in Hardy-Weinberg equilibrium. The MTHFR 677TT genotype was more frequent in patients from dDM and dnD groups compared with control subjects (OR[sub dDM]=2.63, 95% CI: 1.39<OR<4.96, OR[sub dnD]=2.0, 95% CI: 1.02<OR<3.91). eNOS 4a/5b also increases the risk for renal failure, but is lower than the risk conferred by MTHFR C677T (OR[sub dDM]=2.18, 95% CI: 0.90<OR<5.27, and OR[sub dnD]=1.91, 95% CI: 0.77<OR<4.77). Regarding ACE DD genotype or D allele, our results are not in agreement with several earlier studies which have indicated a strong association with ESRD in some Caucasian populations (OR ∼1). This study showed no association between the presence of ACE DD genotype or D allele and ESRD in Romanian patients. In conclusion our data demonstrate: 1. The MTHFR C677T and eNOS 4a/5b can predispose to the development of ESRD in diabetic and nondiabetic patients. The higher risk was observed for MTHFR 677TT genotype in diabetic patients. 2. ACE ID genotype may only have a weak contribution to ESRD development.
- Subjects
ROMANIA; PEOPLE with diabetes; CHRONIC kidney failure; ACE inhibitors; NITRIC oxide; GENETIC polymorphisms
- Publication
Diabetes, 2007, Vol 56, pA92
- ISSN
0012-1797
- Publication type
Article