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- Title
Polymer Translocation and Nanopore Sequencing: A Review of Advances and Challenges.
- Authors
Singh, Swarn Lata; Chauhan, Keerti; Bharadwaj, Atul S.; Kishore, Vimal; Laux, Peter; Luch, Andreas; Singh, Ajay Vikram
- Abstract
Various biological processes involve the translocation of macromolecules across nanopores; these pores are basically protein channels embedded in membranes. Understanding the mechanism of translocation is crucial to a range of technological applications, including DNA sequencing, single molecule detection, and controlled drug delivery. In this spirit, numerous efforts have been made to develop polymer translocation-based sequencing devices, these efforts include findings and insights from theoretical modeling, simulations, and experimental studies. As much as the past and ongoing studies have added to the knowledge, the practical realization of low-cost, high-throughput sequencing devices, however, has still not been realized. There are challenges, the foremost of which is controlling the speed of translocation at the single monomer level, which remain to be addressed in order to use polymer translocation-based methods for sensing applications. In this article, we review the recent studies aimed at developing control over the dynamics of polymer translocation through nanopores.
- Subjects
SINGLE molecule detection; NUCLEOTIDE sequencing; DNA sequencing; MACROMOLECULES
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 7, p6153
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms24076153