We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Otoprotective Effects of Fucoidan Reduce Cisplatin-Induced Ototoxicity in Mouse Cochlear UB/OC-2 Cells.
- Authors
Hsieh, Cheng-Yu; Lin, Jia-Ni; Kang, Ting-Ya; Wen, Yu-Hsuan; Yu, Szu-Hui; Wu, Chen-Chi; Wu, Hung-Pin
- Abstract
Cisplatin is a widely used standard chemotherapy for various cancers. However, cisplatin treatment is associated with severe ototoxicity. Fucoidan is a complex sulfated polysaccharide mainly derived from brown seaweeds, and it shows multiple bioactivities such as antimicrobial, anti-inflammatory, anticancer, and antioxidant activities. Despite evidence of the antioxidant effects of fucoidan, research on its otoprotective effects remains limited. Therefore, the present study investigated the otoprotective effects of fucoidan in vitro using the mouse cochlear cell line UB/OC-2 to develop new strategies to attenuate cisplatin-induced ototoxicity. We quantified the cell membrane potential and analyzed regulators and cascade proteins in the apoptotic pathway. Mouse cochlear UB/OC-2 cells were pre-treated with fucoidan before cisplatin exposure. The effects on cochlear hair cell viability, mitochondrial function, and apoptosis-related proteins were determined via flow cytometry, Western blot analysis, and fluorescence staining. Fucoidan treatment reduced cisplatin-induced intracellular reactive oxygen species production, stabilized mitochondrial membrane potential, inhibited mitochondrial dysfunction, and successfully protected hair cells from apoptosis. Furthermore, fucoidan exerted antioxidant effects against oxidative stress by regulating the Nrf2 pathway. Therefore, we suggest that fucoidan may represent a potential therapeutic agent for developing a new otoprotective strategy.
- Subjects
HAIR cells; OTOTOXICITY; WESTERN immunoblotting; MEMBRANE potential; REACTIVE oxygen species; MITOCHONDRIAL membranes
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 4, p3561
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms24043561