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- Title
Coronin 1 Is Required for Integrin β2 Translocation in Platelets.
- Authors
Riley, David R. J.; Khalil, Jawad S.; Pieters, Jean; Naseem, Khalid M.; Rivero, Francisco
- Abstract
Remodeling of the actin cytoskeleton is one of the critical events that allows platelets to undergo morphological and functional changes in response to receptor-mediated signaling cascades. Coronins are a family of evolutionarily conserved proteins implicated in the regulation of the actin cytoskeleton, represented by the abundant coronins 1, 2, and 3 and the less abundant coronin 7 in platelets, but their functions in these cells are poorly understood. A recent report revealed impaired agonist-induced actin polymerization and cofilin phosphoregulation and altered thrombus formation in vivo as salient phenotypes in the absence of an overt hemostasis defect in vivo in a knockout mouse model of coronin 1. Here we show that the absence of coronin 1 is associated with impaired translocation of integrin β2 to the platelet surface upon stimulation with thrombin while morphological and functional alterations, including defects in Arp2/3 complex localization and cAMP-dependent signaling, are absent. Our results suggest a large extent of functional overlap among coronins 1, 2, and 3 in platelets, while aspects like integrin β2 translocation are specifically or predominantly dependent on coronin 1.
- Subjects
INTEGRINS; BLOOD platelets; CELL physiology; KNOCKOUT mice; CYTOSKELETON; CHROMOSOMAL translocation
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 1, p356
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21010356