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- Title
Discovery of Novel Potential Reversible Peptidyl Arginine Deiminase Inhibitor.
- Authors
Aliko, Ardita; Kamińska, Marta; Falkowski, Katherine; Bielecka, Ewa; Benedyk-Machaczka, Malgorzata; Malicki, Stanisław; Kozieł, Joanna; Wong, Alicia; Bryzek, Danuta; Kantyka, Tomasz; Mydel, Piotr
- Abstract
Citrullination, a posttranslational modification, is catalyzed by peptidylarginine deiminases (PADs), a unique family of enzymes that converts peptidyl-arginine to peptidyl-citrulline. Overexpression and/or increased PAD activity is observed in rheumatoid arthritis (RA), Alzheimer's disease, multiple sclerosis, and cancer. Moreover, bacterial PADs, such as Porphyromonas gingivalis PAD (PPAD), may have a role in the pathogenesis of RA, indicating PADs as promising therapeutic targets. Herein, six novel compounds were examined as potential inhibitors of human PAD4 and PPAD, and compared to an irreversible PAD inhibitor, Cl-amidine. Four of the tested compounds (compounds 2, 3, 4, and 6) exhibited a micromolar-range inhibition potency against PAD4 and no effect against PPAD in the in vitro assays. Compound 4 was able to inhibit the PAD4-induced citrullination of H3 histone with higher efficiency than Cl-amidine. In conclusion, compound 4 was highly effective and presents a promising direction in the search for novel RA treatment strategies.
- Subjects
PORPHYROMONAS gingivalis; RHEUMATOID arthritis; MULTIPLE sclerosis; AMIDINES; HISTONES
- Publication
International Journal of Molecular Sciences, 2019, Vol 20, Issue 9, p2174
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms20092174