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- Title
Resistance Training Regulates Cardiac Function through Modulation of miRNA-214.
- Authors
Soares Melo, Stéphano Freitas; Garrone Barauna, Valério; Carneiro Júnior, Miguel Araújo; Marchesi Bozi, Luiz Henrique; Rios Drummond, Lucas; José Natali, Antônio; de Oliveira, Edilamar Menezes
- Abstract
Aims: To determine the effects of resistance training (RT) on the expression of microRNA (miRNA)-214 and its target in sarcoplasmic reticulum Ca2+-ATPase (SERCA2a), and on the morphological and mechanical properties of isolated left ventricular myocytes. Main methods: Male Wistar rats were divided into two groups (n = 7/group): Control (CO) or trained (TR). The exercise-training protocol consisted of: 4 × 12 bouts, 5×/week during 8 weeks, with 80% of one repetition maximum. Key findings: RT increased the left ventricular myocyte width by 15% and volume by 12%, compared with control animals (p < 0.05). The time to half relaxation and time to peak were 8.4% and 4.4% lower, respectively, in cells from TR group as compared to CO group (p < 0.05). RT decreased miRNA-214 level by 18.5% while its target SERCA2a expression were 18.5% higher (p < 0.05). Significance: Our findings showed that RT increases single left ventricular myocyte dimensions and also leads to faster cell contraction and relaxation. These mechanical adaptations may be related to the augmented expression of SERCA2a which, in turn, may be associated with the epigenetic modification of decreased miRNA-214 expression.
- Subjects
RESISTANCE training; HEART function tests; HEART physiology; MICRORNA; ADENOSINE triphosphatase; GENE expression
- Publication
International Journal of Molecular Sciences, 2015, Vol 16, Issue 4, p6855
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms16046855