We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Necrostatin-1 Attenuates Ischemia Injury Induced Cell Death in Rat Tubular Cell Line NRK-52E through Decreased Drp1 Expression.
- Authors
Li Zhang; Fen Jiang; Yuanhan Chen; Jialun Luo; Shuangxin Liu; Bin Zhang; Zhiming Ye; Wenjian Wang; Xinling Liang; Wei Shi
- Abstract
Necrostatin-1 (Nec-1) inhibits necroptosis and is usually regarded as having no effect on other cell deaths. Here, this study explored whether the addition of Nec-1 has an effect on cell death induced by simulated ischemia injury in rat tubular cell line NRK-52E. In addition, we also investigated the mechanism of Nec-1 attenuates cell death in this renal ischemia model. The NRK-52E cells were incubated with TNF-α + antimycinA (TA) for 24 h with or without Nec-1. Cell death was observed under fluorescent microscope and quantified by flow cytometry. Cell viabilities were detected by MTT assay. The protein expression of dynamin-related protein 1 (Drp1) was detected by Western blotting and immunofluorescence assay. Increased cell death in simulated ischemia injury of NRK-52E cells were markedly attenuated in the Nec-1 pretreated ischemia injury group. Meanwhile, cell viability was significantly improved after using Nec-1. In addition, we also observed that the protein expression of Drp1, a mediator of mitochondrial fission, was significantly increased in simulated ischemia injury group. Increased Drp1 expression in the ischemia injury group can be abolished by Nec-1 or Drp1-knock down, accompanied with decreased cell death and improved cell viabilities. These results suggest that Nec-1 may inhibit cell death induced by simulated ischemia injury in the rat tubular cell line NRK-52E through decreased Drp1 expression.
- Subjects
CELL death; ISCHEMIA; CYTOMETRY; CELLS; IMMUNOFLUORESCENCE
- Publication
International Journal of Molecular Sciences, 2013, Vol 14, Issue 12, p24742
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms141224742