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- Title
Development of Nine Markers and Characterization of the Microsatellite Loci in the Endangered Gymnogobius isaza (Gobiidae).
- Authors
Araki, Kiwako S.; Nakazawa, Takefumi; Kawakita, Atsushi; Kudoh, Hiroshi; Okuda, Noboru
- Abstract
Gymnogobius isaza is a freshwater goby endemic to Lake Biwa, Japan. They experienced a drastic demographic bottleneck in the 1950s and 1980s and slightly recovered thereafter, but the population size is still very small. To reveal dynamics of genetic diversity of G. isaza, we developed nine microsatellite markers based on the sequence data of a related goby Chaenogobius annularis. Nine SSR (Simple Sequence Repeats) markers were successfully amplified for raw and formalin-fixed fish samples. The number of alleles and expected heterozygosities ranged from one to 10 and from 0.06 to 0.84, respectively, for the current samples, while one to 12 and 0.09 to 0.83 for historical samples. The markers described here will be useful for investigating the genetic diversity and gene flow and for conservation of G. isaza.
- Subjects
GENETIC markers; MICROSATELLITE repeats; GYMNOGOBIUS; FISH genetics; FISH diversity; NUCLEOTIDE sequence
- Publication
International Journal of Molecular Sciences, 2012, Vol 13, Issue 5, p5700
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms13055700