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- Title
Isolation and Purification of a Novel Deca-Antifungal Peptide from Potato (Solanum tuberosum L. cv. Jopung) Against Candida albicans.
- Authors
Jong-Kook Lee; Gopal, Ramamourthy; Chang Ho Seo; Hyeonsook Cheong; Yoonkyung Park
- Abstract
In a previous study, an antifungal protein, AFP-J, was purified from tubers of the potato (Solanum tuberosum cv. L Jopung) and by gel filtration and HPLC. In this study, the functional peptide was characterized by partial acid digestion using HCl and HPLC. We obtained three peaks from the AFP-J, the first and third peaks were not active in the tested fungal strain. However, the second peak, which was named Potide-J, was active (MIC; 6.25 µg/mL) against Candida albicans. The amino acid sequences were analyzed by automated Edman degradation, and the amino acid sequence of Potide-J was determined to be Ala-Val-Cys-Glu-Asn-Asp-Leu-Asn-Cys-Cys. Mass spectrometry showed that its molecular mass was 1083.1 Da. Finally, we confirmed that a disulfide bond was present between Cys³ and Cys9 or Cys10. Using this structure, Potide-J was synthesized via solid-phase methods. In these experiments, only the linear sequence was shown to display strong activity against Candida albicans. These results suggest that Potide-J may be an excellent candidate compound for the development of commercially applicable antibiotic agents.
- Subjects
POTATO disease &; pest resistance; ANTIFUNGAL agents; PEPTIDES; CANDIDA albicans; PLANT species; HIGH performance liquid chromatography; PATHOGENIC fungi
- Publication
International Journal of Molecular Sciences, 2012, Vol 13, Issue 4, p4021
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms13044021