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- Title
Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays.
- Authors
Giraud, Gerard; Schulze, Holger; Bachmann, Till T.; Campbell, Colin J.; Mount, Andrew R.; Ghazal, Peter; Khondoker, Mizanur R.; Ross, Alan J.; Ember, Stuart W. J.; Ciani, Ilenia; Tlili, Chaker; Walton, Anthony J.; Terry, Jonathan G.; Crain, Jason
- Abstract
Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime value of 18.8 ns, compared to 13.3 ns obtained for spots of free QD solution, revealing that QD labels are sensitive to the spot microenvironment. Additionally, time gated detection was shown to improve the microarray image contrast ratio by 1.8, achieving femtomolar target sensitivity. Finally, lifetime multiplexing based on Qdot525 and Alexa430 was demonstrated using a single excitation-detection readout channel.
- Subjects
NUCLEIC acids; DEOXYRIBOSE; QUANTUM dots; BIOCHIPS; IMMOBILIZED nucleic acids; TOXICOGENOMICS; DNA microarrays; NUCLEIC acid hybridization; MICROBIAL genetics; MEDICAL research
- Publication
International Journal of Molecular Sciences, 2009, Vol 10, Issue 4, p1930
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms10041930