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- Title
Anti-tumor effects of Astragalus on hepatocellular carcinoma in vivo.
- Authors
Lian-Kun Li; Wen-Juan Kuang; Yun-Feng Huang; Han-Hong Xie; Guo Chen; Qing-Chun Zhou; Bin-Rong Wang; Li-Hong Wan
- Abstract
Objective: The objective of the present study is to investigate the anti-proliferation activity of Astragalus on human hepatocellular carcinoma (HCC) cells and its mechanism. Materials and Methods: Hepatic cancer H22 bearing mice were used to study the antihepatocarcinoma activity of Astragalus in vivo. The growth curve and inhibitory rate of tumor growth were measured. Cell apoptosis of each group was measured by flow cytometry (FCM). Protein expression of Bax and Bcl-2 were analyzed by immunohistochemistry (IHC). The Statistical Package for Social Sciences version 13.0 (SPSS Inc, Chicago, IL) was used for standard statistical analysis including one-way ANOVA and Student's t-test. A value of P<0.05 was considered to be statistically significant. Results: Astragalus significantly inhibited the growth of H22 carcinoma, with an inhibitory rate of 17.28-52.36%. FCM and immunohistochemical assay show that the cell apoptosis rate and protein expression of Bax and Bax/Bcl-2 ratio of H22 transplanted tumor in Astragalus treated group were significantly higher than the control group (P<0.05). The protein expression of Bcl-2 was significantly lower than control (P<0.05). Conclusion: The results of the present study suggest that Astragalus has significant antitumor effect in vivo in inducing apoptosis of H22 tumor cells by promoting protein expression of Bax, decreasing protein expression of Bcl-2 gene, and markedly increasing the Bax/Bcl-2 ratio.
- Subjects
ANTINEOPLASTIC agents; LIVER cancer; CELL death; FLOW cytometry; LABORATORY rats; IMMUNOHISTOCHEMISTRY
- Publication
Indian Journal of Pharmacology, 2012, Vol 44, Issue 1, p78
- ISSN
0253-7613
- Publication type
Article
- DOI
10.4103/0253-7613.91872