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- Title
Hemagglutinin-33 of type A botulinum neurotoxin complex binds with synaptotagmin II.
- Authors
Zhou, Yu; Foss, Sean; Lindo, Paul; Sarkar, Hemanta; Singh, Bal Ram
- Abstract
Botulinum neurotoxin type A (BoNT/A), the most toxic substance known to mankind, is produced byClostridium botulinumtype A as a complex with a group of neurotoxin-associated proteins (NAPs) through polycistronic expression of a clustered group of genes. NAPs are known to protect BoNT against adverse environmental conditions and proteolytic digestion. Hemagglutinin-33 (Hn-33) is a 33 kDa subcomponent of NAPs that is resistant to protease digestion, a feature likely to be involved in the protection of the botulinum neurotoxin from proteolysis. However, it is not known whether Hn-33 plays any role other than the protection of BoNT. Using immunoaffinity column chromatography and pull-down assays, we have now discovered that Hn-33 binds to synaptotagmin II, the putative receptor of botulinum neurotoxin. This finding provides important information relevant to the design of novel antibotulism therapeutic agents targeted to block the entry of botulinum neurotoxin into nerve cells.
- Subjects
BOTULINUM toxin; BACTERIAL toxins; CLOSTRIDIUM botulinum; HEMAGGLUTININ; BLOOD agglutination; SYNAPTOSOMES; BIOCHEMISTRY
- Publication
FEBS Journal, 2005, Vol 272, Issue 11, p2717
- ISSN
1742-464X
- Publication type
Article
- DOI
10.1111/j.1742-4658.2005.04688.x