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- Title
MICROBIOLOGICAL ASSAY OF VITAMIN B<sub>12</sub> CONTENT OF SERUM USING EUGLENA GRACILIS.
- Authors
Nicholas, Deirdre R.; Pitney, W. R.
- Abstract
This article describes the microbiological assay of vitamin B12 content of serum using Euglenal gracilis. Vitamin B12 can be replaced by a few other naturally occurring substances as a growth requirement for Euglena, but these have not been found in serum. Ochromonas malhamensis is more obligatory in its requirements for vitamin B12 than Euglena gracilis. Mollin and Ross (1955) found comparable values for vitamin B12 in serum using both Euglena gracilis and Ochroinonas malhamensis. Euglena assay has some technical advantages over methods using other organisms. Small quantities only of serum are required for assay and these need no preliminary treatment.
- Subjects
MICROBIOLOGICAL assay; SERUM; BLOOD plasma; VITAMIN B12; EUGLENA gracilis; OCHROMONAS malhamensis
- Publication
Australian Journal of Experimental Biology & Medical Science, 1958, Vol 36, Issue 6, p603
- ISSN
0004-945X
- Publication type
Article
- DOI
10.1038/icb.1958.65