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- Title
Heterologous expression of the lectin CmRlec from Cordyceps militaris (Cordycipitaceae, Ascomycota) in Escherichia coli.
- Authors
Akiko Ono; Jili Zhang; Yuki Tanaka; Chihiro Sato; Akiyoshi Yoda; Makoto Ogata; Takahito Nomura; Tomohiro Suzuki
- Abstract
Ascomycete lectins may play an important role in their life cycle. In this report, we mined a ricin B-type lectin, named CmRlec, from the Cordyceps militaris genome by homology search. Furthermore, we succeeded in the soluble expression of CmRlec using β-glucuronidase as a solubilization tag and demonstrated that this lectin is a novel chitin-recognizing lectin.
- Subjects
CORDYCEPS; ESCHERICHIA coli; LIFE cycles (Biology); RICIN; LECTINS; ASCOMYCETES
- Publication
Bioscience, Biotechnology & Biochemistry, 2023, Vol 87, Issue 7, p742
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbad045