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- Title
Quantification of the expression level of integrin receptor α<sub>v</sub>β<sub>3</sub> in cell lines and MR imaging with antibody-coated iron oxide particles.
- Authors
Benedetto, Sabrina; Pulito, Roberta; Crich, Simonetta Geninatti; Tarone, Guido; Aime, Silvio; Silengo, Lorenzo; Hamm, Jörg
- Abstract
Targeted imaging requires site-specific accumulation of a contrast agent (CA), and the properties of that agent must be selected according to the abundance of the target to obtain a signal above the detection limit of the instrument. However, numerical estimates of receptors per cell are rarely found in the literature. Integrin receptors would be particularly promising targets because of their accessibility from the blood stream and expression on activated neovascular endothelial cells. We systematically estimated the number of integrin receptors of cell lines and primary cells by flow cytometry analysis. Since integrin receptors are heterodimeric molecules, and αv forms complexes with various β subunits, the numbers of αv and β3 subunits are therefore dissimilar. The observed values are 3 · 103-1.4 · 104/cell for αv, and 5.3 · 102-1.1 · 104/cell for β3. Despite the low number of exposed receptors, we show that up to single-cell MR visualization can be achieved with the use of iron oxide beads complexed with antibodies as CAs. Magn Reson Med, 2006. © 2006 Wiley-Liss, Inc.
- Publication
Magnetic Resonance in Medicine, 2006, Vol 56, Issue 4, p711
- ISSN
0740-3194
- Publication type
Article
- DOI
10.1002/mrm.21023