We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
La<sup>3+</sup> stimulate the activity of calcineurin in two different ways.
- Authors
Jian Hu; Xiaoda Yang; Kui Wang
- Abstract
It is well known that the activity of calcineurin (CaN) could be modulated by several transitional metal ions. In the present work, the effects of a calcium analog, lanthanum ion (La3+), on the activity of CaN were studied. It was found that La3+ exerted multiple effects on CaN activity. La3+ could stimulate CaN in the absence of calmodulin (CaM); whereas at low concentrations of La3+, there was a slight inhibition of activation of CaN in the presence of CaM. Competitive experiments and limited trypsin proteolysis confirmed that La3+ did not act on the catalytic core of CaN, but exerted its effect through direct action on the CaN regulatory domain similar to Mg2+. In activity titration and spot blotting studies, La3+-containing CaM complexes were less effective in stimulating CaN than Ca2+ or Mn2+-containing CaM; however, the binding affinity of these metal–CaM complexes to CaN was similar. These effects of La3+ on CaN activity are unique among metal ions and may provide clues to understand the biological effects of La3+.
- Subjects
IONS; PROPERTIES of matter; INTERMEDIATES (Chemistry); DIGESTIVE enzymes; PANCREATIC secretions; VOLUMETRIC analysis
- Publication
Journal of Biological Inorganic Chemistry (JBIC), 2005, Vol 10, Issue 6, p704
- ISSN
0949-8257
- Publication type
Article
- DOI
10.1007/s00775-005-0021-5