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- Title
Isolation and characterization of a newly identified Clostridium butyricum strain SCUT343-4 for 1,3-propanediol production.
- Authors
Lan, Yang; Feng, Jun; Guo, Xiaolong; Fu, Hongxin; Wang, Jufang
- Abstract
A novel 1,3-propanediol (1,3-PDO) producing strain was isolated and identified as Clostridium butyricum with respect to its morphological and physiological characteristics, as well as 16S rDNA. The results of substrates test and stress tolerance indicated that C. butyricum SCUT343-4 could produce 1,3-PDO efficiently from glycerol. The optimal fermentation conditions were determined to be 5 g/L yeast extract at 37 °C and pH 6.5. To fully evaluate its 1,3-PDO production capacity, different cultivation strategies have been implemented. The highest 1,3-PDO concentration obtained for batch and fed-batch fermentation were 51.64 and 61.30 g/L, respectively. Immobilized cell fermentation in fibrous-bed bioreactor was also performed, and the concentration of 1,3-PDO further increased to 86 g/L with a yield of 0.52 g/g. In addition, the 1,3-PDO productivity reached 4.20 g/L h, which is the highest level reported for C. butyricum, demonstrating the potential of C. butyricum SCUT343-4 for 1,3-PDO production from glycerol.
- Subjects
CLOSTRIDIUM butyricum; CLOSTRIDIUM acetobutylicum; IMMOBILIZED cells; YEAST extract; CLOSTRIDIUM perfringens; GLYCERIN; INDUSTRIAL capacity; FERMENTATION
- Publication
Bioprocess & Biosystems Engineering, 2021, Vol 44, Issue 11, p2375
- ISSN
1615-7591
- Publication type
Article
- DOI
10.1007/s00449-021-02610-x