We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Apoptosis of odontoclasts under physiological root resorption of human deciduous teeth.
- Authors
Domon, Takanori; Taniguchi, Yumi; Inoue, Kiichiro; Ushijima, Natsumi; Taishi, Yoshihito; Hiramatsu, Akiko; Wakita, Minoru; Yoshida, Shigemitsu
- Abstract
This study was designed to establish the apoptosis of odontoclasts during physiological root resorption of human deciduous teeth. Deciduous teeth were fixed, decalcified, and embedded in paraffin for immunohistochemical (IHC) observations and in Epon for transmission electron microscopy (TEM). Apoptotic cells were identified by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick-end labeling (TUNEL), and then tartrate-resistant acid phosphatase (TRAP) activity was determined on the same sections. Epon-embedded specimens were sectioned serially into 0.5-μm semithin sections; some of these sections were re-embedded in Epon, sectioned into 0.1-μm ultrathin sections, and observed by TEM. IHC revealed that the nuclei of TRAP-positive odontoclasts on the dentine were generally TUNEL-negative. Around these odontoclasts, a few TRAP-positive structures were present together with TUNEL-positive structures, e.g., a TRAP-positive structure with one TUNEL-positive nucleus, a TRAP-positive structure with one TUNEL-positive nucleus plus one or two TUNEL-negative nuclei, or a TRAP-positive structure with no nucleus. By TEM, some odontoclasts showed nuclear fragments including compacted chromatin. The results suggest that, during apoptosis, odontoclasts fragment into variously sized cellular parts including three or fewer nuclei.
- Subjects
APOPTOSIS; ODONTOCLASTS; DECIDUOUS teeth; IMMUNOHISTOCHEMISTRY; CELL death; CELL culture; CELL lines; TEETH; CYTOLOGICAL research; TISSUES
- Publication
Cell & Tissue Research, 2008, Vol 331, Issue 2, p423
- ISSN
0302-766X
- Publication type
Article
- DOI
10.1007/s00441-007-0525-0