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- Title
A REAL-TIME VIEW OF LIFE WITHIN 100 NM OF THE PLASMA MEMBRANE.
- Authors
Steyer, J. A.; Almers, W.
- Abstract
The plasma membrane is a two-dimensional compartment that relays most biological signals sent or received by a cell. Signalling involves membrane receptors and their associated enzyme cascades as well as organelles such as exocytic and endocytic vesicles. Advances in light microscope design, new organelle-specific vital stains and fluorescent proteins have renewed the interest in evanescent field fluorescence microscopy, a method uniquely suited to image the plasma membrane with its associated organelles and macromolecules in living cells. The method shows even the smallest vesicles made by cells, and can image the dynamics of single protein molecules.
- Subjects
CELL membranes; BIOLOGICAL membranes; FLUORESCENCE microscopy; MOLECULAR biology; CELL physiology
- Publication
Nature Reviews Molecular Cell Biology, 2001, Vol 2, Issue 4, p268
- ISSN
1471-0072
- Publication type
Article
- DOI
10.1038/35067069