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- Title
Bélrendszeri károsodást jelző biomarkerek vizsgálata Isosporafertőzött kutyákban.
- Authors
Yıldız, Ibrahim Kubilay; Ok, Mahmut
- Abstract
This study aimed to investigate the potential efficacy of selected intestinal damage biomarkers in determination of the presence of intestinal epithelial damage in dogs with isosporiasis. Animal material of this study consisted of 40 dogs in total, aged 2-6 months old from different breeds, both sexes. Of these, 30 were infected with isosporiasis (Trial Group) and 10 were healthy (Control Group). Fatigue, depression, fever, vomiting, abdominal pain, and mucoid to hemorrhagic diarrhea were observed in the Trial Group. The diagnosis was confirmed by the presence of Isospora oocytes under microscopic examination of fecal samples of dogs suspected of being affected with isosporiasis. Blood samples were taken from all dogs via v. cephalica or v. cephana venepunction.and hemogram analysis was performed using autoanalyzer from blood samples with anticoagulant (K3EDTA). For biomarker measurements, blood samples with coagulant were used. Serum intestinal-fatty acid-binding protein (I-FABP), trefoil factor 3 (TFF3), intestinal alkaline phosphatase (IAP), and gamma-enteric smooth muscle actin (ACTG2) levels were measured with ELISA kits. As a result of standard treatment protocol, 28 of 30 dogs with isosporiasis recovered while two of them died. Results of hemogram analysis showed that leukocytes (WBC) and granulocytes (Gran) levels were increased whereas erythrocytes (RBC), hematocrit (Hct), and hemoglobin (Hgb) levels were decreased. Moreover, serum I-FABP, IAP, TFF3, and ACTG2 levels were found to be significantly higher in dogs with isosporiasis compared to the healthy ones. In conclusion, this study showed a significant increase in serum I-FABP, IAP, TFF3, and ACTG2 levels in dogs with isosporiasis. Also, these biomarkers were found to be useful and reliable in determination of intestinal epithelial damage caused by isosporiasis in dogs. This manuscript was summarized from M.Sc. thesis of İbrahim Kubilay Yıldız.
- Subjects
TREFOIL factors; ALKALINE phosphatase; SMOOTH muscle; BLOOD cell count; BLOOD sampling
- Publication
Magyar Állatorvosok Lapja, 2022, Vol 144, Issue 2, p101
- ISSN
0025-004X
- Publication type
Article