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- Title
A reliable and efficient BioPulverizer method in preparing and grinding nematodes for nucleic acid extraction and molecular identification.
- Authors
Ye Jiang; Ruifeng Qin; Chunjie Li; Minghui Huang; Dan Jiang; Doudou Chang; Yifan Xie; Yanan Zhao; Congli Wang
- Abstract
Due to their tiny size, soil nematodes and plant-parasitic nematodes are challenging to homogenize and collect using traditional mortar and pestle methods. To overcome this, we developed a reliable and efficient nematode preparation and grinding method using the BioPulverizer. The method involves creating a modified sample 'sandwich' with liquid nitrogen-frozen nematode samples placed between two layers of aluminum foil. This 'sandwich' facilitates complete homogenization through cryogenic grinding without any sample loss during transfer. The powerful nematode grinding yields high-quality and high-quantity samples, suitable for nucleic acid release and subsequent molecular identification and other downstream applications.
- Subjects
NUCLEIC acids; SOIL nematodes; CRYOGENIC grinding; ALUMINUM foil; PLANT nematodes; NEMATODES
- Publication
Zoologia, 2023, Vol 40, p1
- ISSN
1984-4670
- Publication type
Article
- DOI
10.1590/S1984-4689.v40.e23021