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- Title
Mechanical Stimulation by Ultrasound Enhances Chondrogenic Differentiation of Mesenchymal Stem Cells in a Fibrin-Hyaluronic Acid Hydrogel.
- Authors
Choi, Jae Won; Choi, Byung Hyune; Park, Sang‐Hyug; Pai, Ki Soo; Li, Tian Zhu; Min, Byoung‐Hyun; Park, So Ra
- Abstract
Chondrogenic differentiation and cartilage tissue formation derived from stem cells are highly dependent on both biological and mechanical factors. This study investigated whether or not fibrin-hyaluronic acid ( HA) coupled with low-intensity ultrasound ( LIUS), a mechanical stimulation, produces an additive or synergistic effect on the chondrogenesis of rabbit mesenchymal stem cells ( MSCs) derived from bone marrow. For the purpose of comparison, rabbit MSCs were first cultured in fibrin- HA or alginate hydrogels, and then subjected to chondrogenic differentiation in chondrogenic-defined medium for 4 weeks in the presence of either transforming growth factor-beta3 ( TGF-β3) (10 ng/mL) or LIUS treatment (1.0 MHz and 200 mW/cm2). The resulting samples were evaluated at 1 and 4 weeks by histological observation, chemical assays, and mechanical analysis. The fibrin- HA hydrogel was found to be more efficient than alginate in promoting chondrogenesis of the MSCs by producing a larger amount of sulfated glycosaminoglycans ( GAGs) and collagen, and engineered constructs made with the hydrogel demonstrated higher mechanical strength. At 4 weeks of tissue culture, the chondrogenesis of the MSCs in fibrin- HA were shown to be further enhanced by treatment with LIUS, as observed by analyses for the amounts of GAGs and collagen, and mechanical strength testing. In contrast, TGF-β3, a well-known chondrogenic inducer, showed a marginal additive effect in the amount of collagen only. These results revealed that LIUS further enhanced chondrogenesis of the MSCs cultured in fibrin- HA, in vitro , and suggested that the combination of fibrin- HA and LIUS is a useful tool in constructing high-quality cartilage tissues from MSCs.
- Subjects
CHONDROGENESIS; CARTILAGE; STEM cells; FIBRIN; HYDROGELS
- Publication
Artificial Organs, 2013, Vol 37, Issue 7, p648
- ISSN
0160-564X
- Publication type
Article
- DOI
10.1111/aor.12041