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- Title
三叶木通愈伤诱导及分化研究.
- Authors
贾明良; 方荷芳; 李同建; 文锋; 韩兴杰; 金洪光; 徐玲玲; 廖亮
- Abstract
Akebia trifoliate (Thunb.) Koidz. has high development and application value, and it is very important to establish the tissue culture system for its excellent strains. Taking the young branches and leaves of Akebia trifoliate (Thunb.) Koidz. as explants, this paper studied the processes of aseptic explants, callus induction and embryoid induction, and analyzed the factors influencing the inhibition of callus browning. The results showed that: after 75% ethanol disinfection of 1 min and mercuric chloride sterilization of 12 min, aseptic explants were obtained. After soaking 3 min in sterile water, then explants were inoculated to the culture medium (MS + NAA 0.4 mg·L-1 +2, 4-D 4.0 mg·L-1 + characoal activited 1.0 g·L-1 + sucrose 30.0 g·L-1 + agar 6.0 g·L-1) culturing without light, callus could be obtained, and the browning of callus could be effectively inhibited. The obtained callus inoculated on the media of MS + NAA 0.4 mg·L-1 +6-BA 2.0 mg·L-1 + sucrose 30.0 g·L-1 + agar 6.0 g·L-1 could obtain the embryos, and then seedlings could be obtained after inoculation into a hormone free MS medium.
- Subjects
MERCURIC chloride; ASEPTIC packaging; TISSUE culture; STERILIZATION (Disinfection); CALLUS; SUCROSE; AGAR
- Publication
Journal of Agricultural Science & Technology (1008-0864), 2020, Vol 22, Issue 3, p181
- ISSN
1008-0864
- Publication type
Article
- DOI
10.13304/j.nykjdb.2019.0123