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- Title
Seminal plasma caspase 3, cytochrome c and total antioxidant capacity in oligospermic males and association with sperm indices.
- Authors
Emokpae, Mathias Abiodun; Chima, Happy Ngozi; Ahmed, Muhammed
- Abstract
Objective: To determine the levels of cytochrome c, caspase 3 and total antioxidant capacity (TAC) in oligospermic male subjects in Zaria, northern Nigeria. Materials and methods: The study participants were 56 oligospermic male subjects attending fertility clinic and 30 control subjects who were males of proven fertility with normal semen parameters. Semen samples were collected by masturbation after a minimum of 3 days' sexual abstinence into sterile containers, were allowed to liquefy at room temperature and manual semen analysis was performed according to WHO standard. Seminal plasma cytochrome c, caspase 3 and total antioxidant capacity were assayed by sandwiched ELISA technique using reagents supplied by Elabscience and Wkea Medical supplies corporation, China respectively. The differences in the mean levels of measured parameters were compared using Student's t test and caspase 3 was correlated with sperm indices. Results: The mean values of cytochrome C and caspase 3 were significantly higher (p<0.001), while TAC was lower (p<0.001) in oligospermia than in the controls. The levels of caspase 3 and cytochrome c were significantly higher in primary than secondary infertile oligospermic subjects. Conclusion: The study showed that biomarkers of mitochondria DNA damage were increased while TAC was decreased in oligospermia and may reflect the fertility potential of spermatozoa.
- Subjects
SEMINAL proteins; CASPASES; ANTIOXIDANTS; OLIGOSPERMIA; SPERMATOZOA
- Publication
Journal of Experimental & Integrative Medicine, 2016, Vol 6, Issue 4, p1
- ISSN
1309-4572
- Publication type
Article
- DOI
10.5455/jeim.091116.or.164