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- Title
The role of ATP-binding cassette transporter genes expression in treatment failure cutaneous leishmaniasis.
- Authors
Boozhmehrani, Mohammad Javad; Eslami, Gilda; Khamesipour, Ali; Jafari, Abbas Ali; Vakili, Mahmood; Hosseini, Saeedeh Sadat; Askari, Vahideh
- Abstract
Leishmaniasis is one of the common diseases transmitted by sand flies in tropical and subtropical regions of the world. Currently, antimonial derivatives are the first line of treatment. Some of the members of the ATP-binding cassette (ABC) family of Leishmania are shown to be associated with no response to treatment. In this study, we evaluated ABCI4, ABCG2, ABCC7, ABCB4, and ABCC3 genes expression in Leishmania isolated from patients with non-healing cutaneous leishmaniasis and treatment response isolates. We selected 17 clinical isolates including 8 treatment failure and 9 treatment response samples from September 2020 to March 2021. The isolates were obtained from patients of Health Center Laboratory of Varzaneh, Isfahan, Iran with cutaneous leishmaniasis. The diagnosis was performed using microscopic observation. The samples were directly collected from the lesions. The expression profiling of genes was assessed using SYBR Green real-time PCR that was analyzed with delta-delta Ct. All treatment failure clinical isolates were L. major. Gene expression analysis in treatment failure isolates showed that the ABC transported genes had a different pattern in each isolate. Treatment failure has been reported for cutaneous leishmaniasis worldwide. Knowledge of the molecular mechanisms of treatment failure could solve this problem. ABC transporter genes are considered controversial over the mechanisms of treatment failure outcomes. In this study, we showed that ABC transporter genes could be considered one of the important mechanisms.
- Subjects
ISFAHAN (Iran); CUTANEOUS leishmaniasis; ATP-binding cassette transporters; LEISHMANIASIS; TREATMENT failure; GENE expression profiling; SAND flies; GENE expression; TREATMENT effectiveness
- Publication
AMB Express, 2022, Vol 12, Issue 1, p1
- ISSN
2191-0855
- Publication type
Article
- DOI
10.1186/s13568-022-01419-5