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- Title
Meta-lens light-sheet fluorescence microscopy for in vivo imaging.
- Authors
Luo, Yuan; Tseng, Ming Lun; Vyas, Sunil; Hsieh, Ting-Yu; Wu, Jui-Ching; Chen, Shang-Yang; Peng, Hsiao-Fang; Su, Vin-Cent; Huang, Tzu-Ting; Kuo, Hsin Yu; Chu, Cheng Hung; Chen, Mu Ku; Chen, Jia-Wern; Chen, Yu-Chun; Huang, Kuang-Yuh; Kuan, Chieh-Hsiung; Shi, Xu; Misawa, Hiroaki; Tsai, Din Ping
- Abstract
Metasurface optics can be utilized for many LSFM modalities to achieve novel functionalities, such as meta-lens arrays [[10]] for light-sheet generation, multi-view/multi-dimensional selective plane illumination microscopy [[47]], near-infrared [[49]] and multi-photon LSFM [[50]]. A bright-field image of the live I C. elegans i is shown in Figure 4a. High-contrast embryo images obtained from the meta-lens LSFM are shown in Figure 4b, while a wide-field fluorescence image is shown in Figure 4c. Figure 4d-f shows corresponding zoom-in images to the dashed-box regions shown in Figure 4a-c. Therefore, the LSFM with the meta-lens can efficiently provide proper illumination for optical sectioning. A scanning electron microscope (SEM) image of the GaN nanopillars in the light-sheet meta-lens and an optical microscope image of the light-sheet meta-lens are shown in Figure 1c and d, respectively. At present, the meta-lens is used only for illumination; however, with further experimentation, the LSFM system, which uses meta-lenses for both the detection and excitation, can be realized.
- Subjects
OPTICAL modulation; OPTICAL elements; NUMERICAL apertures; IMAGING systems; MICROSCOPY; FLUORESCENCE microscopy
- Publication
Nanophotonics (21928606), 2022, Vol 11, Issue 9, p1949
- ISSN
2192-8606
- Publication type
Article
- DOI
10.1515/nanoph-2021-0748