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- Title
Usefulness of 11C-choline positron emission tomography for genital chlamydial infection assessment in a BALB/c murine model.
- Authors
Marangoni, Antonella; Nanni, Cristina; Quarta, Carmelo; Foschi, Claudio; Russo, Incoronata; Nardini, Paola; D'Errico, Antonietta; Rosini, Francesca; Ferretti, Alice; Aldini, Rita; Cevenini, Roberto; Rubello, Domenico
- Abstract
<bold>Purpose: </bold>The aim of this study is to explore the feasibility of 11C-Choline PET in the assessment of the degree of inflammation in the Chlamydia muridarum genital infection model.<bold>Procedures: </bold>Forty female Balb/c mice received 2.5 mg of medroxyprogesterone acetate i.m. 9 and 2 days prior to the infection: 21 mice were infected by C. muridarum into the vaginal vault, 12 mice were treated with inactivated chlamydiae, and 7 mice were SPG buffer-treated as negative controls. Three healthy control mice were not treated with progesterone. Mice in each category were randomly subdivided in two groups: (1) sacrificed at 5, 10, 15, and 20 days for histological analysis and (2) undergoing 11C-Choline PET at days 5, 10, and 20 post-infection (20 MBq of 11C-Choline, uptake time of 10 min, acquisition through a small-animal PET tomograph for 15 min).<bold>Results: </bold>Infected animals showed a significantly higher standardized uptake value than both controls and animals inoculated with heat-inactivated chlamydiae in each PET scan (P<0.05). All organs of the infected animals had scores of inflammation ranging between 2 and 3 at day 5, decreasing to 1-2 at day 20.<bold>Conclusions: </bold>This preliminary result demonstrated that 11C-Choline PET can highlight a specific proliferation mechanism of inflammatory cells induced by C. muridarum, thanks to a very high sensitivity in detecting very small amounts of tracer in inflammatory cells.
- Publication
Molecular Imaging & Biology, 2013, Vol 15, Issue 4, p450
- ISSN
1536-1632
- Publication type
journal article
- DOI
10.1007/s11307-013-0612-4