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- Title
Effects of Preculture on Cryopreservation of Nodes from Dioscorea opposita Thunb. cv. Tiegun Plantlets by Encapsulation-vitrification.
- Authors
Xiting ZHAO; Ping LI; Liwei JIANG; Yuting ZHU; Zhihui SONG; Linlin HAN; Mingjun LI
- Abstract
[Objective] To research the effects of preculture on the cryopreservation by encapsulation-vitrification of Dioscorea opposita Thunb. cv. Tiegun. [Methods] Tissue culture method was adopted; all the preculture tests were carried out under4 °C. [Results] Sucrose concentration ' penetrant types ' preculture time and preculture method had significant impacts on the relative survival rate and regeneration rate of D. opposita nodes after cryopreservation by encapsulation-vitrification. The appropriate sucrose concentration and precultured time were 0. 2 mol/L and 3 d, respectively. When 0.1 mol L sucrose + 0.1 molL glycerol was taken as the penetrant, the relative survival rate and regeneration rate were the maximum after cryopreservation by encapsulation-vitrification. Effect of direct preculture for 3 d on MS+0.1 mol/L sucrose + 0. mol/L glycerol was the best, which could obtain the highest relative survival rate (89%) and regeneration rate (52%) after cryopreservation. Therefore, plantet nodes of D. opp. Thunb. cv. Tiegun should be precurtured on MS + 0.1 mol/L sucrose + 0.1 mol/L glycerol for 3 d at 4 °C. [Conclusions] This research provides a technology system for vitrification, and the method is simple and effective.
- Subjects
YAMS; CRYOPRESERVATION of organs, tissues, etc.; ENCAPSULATION (Catalysis); VITRIFICATION; NODES (Botany); THERAPEUTICS
- Publication
Medicinal Plant, 2016, Vol 7, Issue 1/2, p22
- ISSN
2152-3924
- Publication type
Article