We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Resistance training in humans and mechanical overload in rodents do not elevate muscle protein lactylation.
- Authors
Mattingly, Madison L.; Ruple, Bradley A.; Sexton, Casey L.; Godwin, Joshua S.; McIntosh, Mason C.; Smith, Morgan A.; Plotkin, Daniel L.; Michel, J. Max; Anglin, Derick A.; Kontos, Nicholas J.; Shengyi Fei; Phillips, Stuart M.; Mobley, C. Brooks; Vechetti, Ivan; Vann, Christopher G.; Roberts, Michael D.
- Abstract
Although several reports have hypothesized that exercise may increase skeletalmuscle protein lactylation, empirical evidence in humans is lacking. Thus, we adopted a multifaceted approach to examine if acute and subchronic resistance training (RT) altered skeletal muscle protein lactylation levels. Inmice, we also sought to examine if surgical ablation-induced plantaris hypertrophy coincided with increases in muscle protein lactylation. To examine acute responses, participants' blood lactate concentrations were assessed before, during, and after eight sets of an exhaustive lower body RT bout (n = 10 trained college-agedmen). Vastus lateralis biopsies were also taken before, 3-h post, and 6-h post-exercise to assess muscle protein lactylation. To identify training responses, another cohort of trained college-aged men (n = 14) partook in 6 weeks of lower-body RT (3x/week) and biopsies were obtained before and following the intervention. Five-month-old C57BL/6 mice were subjected to 10 days of plantaris overload (OV, n = 8) or served as age-matched sham surgery controls (Sham, n = 8). Although acute resistance training significantly increased blood lactate responses ~7.2-fold (p < 0.001), cytoplasmic and nuclear protein lactylation levels were not significantly altered at the post-exercise time points, and no putative lactylation-dependent mRNA was altered following exercise. Six weeks of RT did not alter cytoplasmic protein lactylation (p = 0.800) despite significantly increasing VLmuscle size (+3.5%, p=0.037), and again, no putative lactylation-dependent mRNA was significantly affected by training. Plantaris muscles were larger in OV versus Sham mice (+43.7%, p < 0.001). However, cytoplasmic protein lactylation was similar between groups (p = 0.369), and nuclear protein lactylation was significantly lower in OV versus Sham mice (p < 0.001). The current null findings, along with other recent null findings in the literature, challenge the thesis that lactate has an appreciable role in promoting skeletalmuscle hypertrophy.
- Subjects
MUSCLE proteins; RESISTANCE training; NUCLEAR proteins; SKELETAL muscle; BLOOD lactate; MONOCARBOXYLATE transporters; VASTUS lateralis
- Publication
Frontiers in Physiology, 2023, p1
- ISSN
1664-042X
- Publication type
Article
- DOI
10.3389/fphys.2023.1281702