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- Title
A Human Full-Skin Culture System for Interventional Studies.
- Authors
Steinstraesser, Lars; Rittig, Andrea; Gevers, Kai; Sorkin, Michael; Hirsch, Tobias; Kesting, Marco; Sand, Michael; Al-Benna, Sammy; Langer, Stefan; Steinau, Hans-Ulrich; Jacobsen, Frank
- Abstract
Objective: Novel approaches to bridge the gap between clinical studies and experimental basic research of skin physiology are urgently needed. The aim of this study was to develop an effective surrogate model in which ex vivo full-thickness organ culture experiments may be performed. Methods: Human full skin from patients was placed into a stainless steel chamber and cultured at an air-liquid interphase for 4 weeks. Samples were evaluated every week by HE-staining and immunohistochemical characterization. Epidermal gene transfer kineticswas performed as an interventional study. Results: This ex vivo chamber model maintained the physiologic and histologic properties of the skin explants for 4 weeks. This indicated the model's acceptable ex vivo physiologic validity. No epidermolysis was observed, and both basal lamina and blood vessels were detected within all tissue samples. Transgene expression was demonstrated to be time dependent. Conclusion: This model chamber presents a convenient, easy-to-use, and robust model in which ex vivo full-thickness organ culture experiments may be performed.
- Subjects
SKIN physiology; ORGAN culture; ORGANS (Anatomy); STAINLESS steel; IMMUNOHISTOCHEMISTRY; PHYSIOLOGY; HISTOLOGY; EPIDERMOLYSIS bullosa; BASAL lamina; TRANSGENE expression
- Publication
ePlasty: Open Access Journal of Plastic Surgery, 2009, Vol 9, p27
- ISSN
1937-5719
- Publication type
Article