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- Title
Short-term storage and cryopreservation ofUrechis unicinctus(Echiura: Urechidae) sperm.
- Authors
Kang, Kyoung Ho; Shao, Ming Yu; Kho, Kang Hee; Zhang, Zhi Feng
- Abstract
In the present study, attempts were made to preserveUrechis unicinctussperm at 4°C. Cryopreservation procedures were optimized for various cryoprotectants and freezing rates, equilibration times and dilution ratios. During short-term storage, the motility of undiluted sperm was extended for 6 days of cold storage,and in 70% and 100% artificial seawater only persisted for 2 and 4 days respectively. The survival rate of undiluted sperm was maintained at a high level accordingly. After cryopreservation, the highest motility and survival rate (41.5±2.2%) were obtained in 15% dimethyl sulphoxide (Me2SO) using a freezing rate of 30°C min−1. After thawing the sperm cryopreserved in glycerol lost almost all motility. The motility and survival rate of post-thawing sperm did not show significant differences after 8 and 15 min equilibration using 15% Me2SO as cryoprotectant; the values were significantly higher than those of 2 min equilibration. Comparisons of motility and survival rate between treatments pooled by dilution ratio showed that the effect of 1:1 ratio (sperm volume to cryoprotectant volume) was best. There was no difference between 1:3 and 1:5, and other ratioswere significantly worse.
- Subjects
URECHIS unicinctus; ECHIURA; URECHIDAE; WORMS; MARINE invertebrates; AQUACULTURE
- Publication
Aquaculture Research, 2004, Vol 35, Issue 13, p1195
- ISSN
1355-557X
- Publication type
Article
- DOI
10.1111/j.1365-2109.2004.01120.x