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- Title
New biomaterial based on cotton with incorporated Biomolecules.
- Authors
Gomes, Ana P.; Mano, João F.; Queiroz, João A.; Gouveia, Isabel C.
- Abstract
ABSTRACT The aim of this study was to investigate a method of embedding l-cysteine ( l-cys), an antimicrobial agent, between layers of chitosan (CH) and sodium alginate (ALG) onto cotton samples obtained via a layer-by-layer electrostatic deposition technique via several embedding methods. The results show that the best way to incorporate l-cys into the layers was the one that used the property of gelling ALG. To monitor the l-cys embedding into the CH/ALG multilayer film, different methods were used: energy-dispersive X-ray spectrometry analysis to assess the presence of sulfur on the sample, Ellman's reagent method to analyze l-cys release from the sample, and attenuated total reflectance (ATR) Fourier transform infrared spectroscopy (FTIR) to compare the ATR-FTIR spectra of the pure l-cys and l-cys embedded in the CH/ALG multilayer film to study the interaction between the l-cys and the CH/ALG multilayer films. Functionalized CH/ALG cotton samples were also investigated for their antibacterial properties toward Staphylococcus aureus and Klebsiella pneumonia with the Japanese Industrial Standard method JIS L 1902:2002, and the results show an enhancement of the antibacterial effect due to the presence of l-cys. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40519.
- Subjects
BIOMATERIALS; COTTON; BIOMOLECULES; CHITOSAN; CYSTEINE; SODIUM alginate; ELECTROSTATICS; FOURIER transform infrared spectroscopy; THERAPEUTICS
- Publication
Journal of Applied Polymer Science, 2014, Vol 131, Issue 15, pn/a
- ISSN
0021-8995
- Publication type
Article
- DOI
10.1002/app.40519