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- Title
Sensitive and Selective Detection of HIV-1 RRE RNA Using Vertical Silicon Nanowire Electrode Array.
- Authors
Jaehyung Lee; Min-Ho Hong; Sanghun Han; Jukwan Na; Ilsoo Kim; Yong-Joon Kwon; Yong-beom Lim; Heon-Jin Choi
- Abstract
In this study, HIV-1 Rev response element (RRE) RNA was detected via an Au-coated vertical silicon nanowire electrode array (VSNEA). The VSNEA was fabricated by combining bottom-up and top-down approaches and then immobilized by artificial peptides for the recognition of HIV-1 RRE. Differential pulse voltammetry (DPV) analysis was used to measure the electrochemical response of the peptide-immobilized VSNEA to the concentration and types of HIV-1 RRE RNA. DPV peaks showed linearity to the concentration of RNA with a detection limit down to 1.513 fM. It also showed the clear different peaks to the mutated HIV-1 RRE RNA. The high sensitivity and selectivity of VSNEA for the detection of HIV-1 RRE RNA may be attributed to the high surface-to-volume ratio and total overlap diffusion mode of ions of the one-dimensional nanowire electrodes.
- Subjects
HIV; RNA; SILICON nanowires; ELECTRODES; PEPTIDES
- Publication
Nanoscale Research Letters, 2016, Vol 11, p1
- ISSN
1931-7573
- Publication type
Article
- DOI
10.1186/s11671-016-1504-8