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- Title
Rapid and simple detection of Glaesserella parasuis in synovial fluid by recombinase polymerase amplification and lateral flow strip.
- Authors
Zhang, Ting-ting; Liu, Meng-zhi; Yin, Rong-huan; Yao, Long-quan; Liu, Bao-shan; Chen, Ze-liang
- Abstract
Background: Glaesserella parasuis (G. parasuis) is an influential pathogen of the pig, which induces high morbidity and mortality in naive pig populations in the pig industry. Accurate and rapid detection of the agent is important for disease control. In this study, a simple recombinase polymerase amplification (RPA) with a Lateral flow (LF) strip (RPA-LF-GPS) was developed to detect G. parasuis. Results: The RPA-LF-GPS can specifically detect G. parasuis a limit of 100 CFU from other common related pathogens causing arthritis in the pig. The RPA-LF-GPS assay can use boiled synovial fluid samples as a template with the same sensitivity as other DNA extraction methods. In the detection of clinic positive synovial fluid sample, RPA-LF-GPS is equally sensitive (98.1%) compared with that of PCR (90.4%) (P > 0.05). The whole procedure of the RPA-LF-GPS assay could be finished in 1 hour without professional equipment. Conclusions: RPA-LF-GPS assay is a rapid and simple method for point-of-care diagnostic testing for G. parasuis infection.
- Subjects
SYNOVIAL fluid; NUCLEIC acid isolation methods; POINT-of-care testing; PREVENTIVE medicine
- Publication
BMC Veterinary Research, 2019, Vol 15, Issue 1, pN.PAG
- ISSN
1746-6148
- Publication type
Article
- DOI
10.1186/s12917-019-2039-x