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- Title
Comparison of RhD Typing Results by Serology and Molecular Methods.
- Authors
Gammon, Richard R; Conceicao, Michelle; Benitez, Nancy; Bright, Frieda; Counts, Kelley; Resto, Claribel; Rexer, Karl
- Abstract
Objective Molecular testing determines D antigen status when abnormal serologic results are observed. Molecular testing is routinely batched, resulting in longer turnaround time for abnormal D status resolution. During the interim, obstetric patients with questionable/uninterpretable and weak D typing results by serology, per the immunohematology reference laboratory (IRL) policy, will receive RhD negative blood. This study aimed to determine whether serology results achieved a concordance. Methods Six hospitals provided samples to the IRL (first IRL) for RhD status by DNA. De-identified samples were sent for serology RhD (second IRL). A concordance of ≥80% was acceptable. Results Forty-nine samples were evaluated. Results were concordant (65.3% [32/49]) and discordant (34.7% [17/49]). This is significantly lower than clinically acceptable 80% (z = 2.57, P < .05). The turnaround-time was 3.0 hours for serology and 4.4 days for molecular evaluation. Conclusion Due to a low concordance, serology could not be used in place of molecular testing.
- Subjects
HOSPITALS; BLOOD grouping &; crossmatching; DNA; SERODIAGNOSIS; ALLELES; MOLECULAR biology; COMPARATIVE studies; RH factor; TURNAROUND time; DESCRIPTIVE statistics; GENETIC markers; RESEARCH funding; OLIGONUCLEOTIDE arrays; GENETIC profile
- Publication
Laboratory Medicine, 2023, Vol 54, Issue 2, p190
- ISSN
0007-5027
- Publication type
Article
- DOI
10.1093/labmed/lmac097