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- Title
Use of Liposomes as an Immunopotentiating Delivery System: in Perspective of Vaccine Development.
- Authors
Owais, M.; Masood, A. K.; Agrewala, J. N.; Bisht, D.; Gupta, C. M.
- Abstract
Liposomes have been widely used to deliver antigens to the antigen-presenting cells (APCs) and also to modify their immunological behaviour in model animals. We recently demonstrated the potential of yeast lipid liposomes to undergo membrane–membrane fusion with cytoplasmic membrane of the target cells. Interestingly, studies in the present report revealed that antigen encapsulated in yeast lipid liposomes could be successfully delivered simultaneously into the cytosolic as well as endosomal processing pathways of APCs, leading to the generation of both CD4+ T helper and CD8+ cytotoxic T cells. In contrast, encapsulation of same antigen in egg phosphatidyl-choline (PC) liposomes, just like its free form, has inefficient access to the cytosolic pathway of major histocompatibility complex (MHC) I dependent antigen presentation and failed to generate antigen specific CD8+ cytotoxic T-cell response. However, both egg PC as well as yeast lipid liposomes have elicited strong antigen specific antibody responses in immunized animals. These results imply usage of liposome encapsulated antigen as potential candidate vaccine capable of eliciting both cell mediated as well as humoral immune responses.
- Subjects
LIPOSOMES; ANTIGENS; YEAST; CELL membranes
- Publication
Scandinavian Journal of Immunology, 2001, Vol 54, Issue 1/2, p125
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1046/j.1365-3083.2001.00944.x