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- Title
T Cell-Mediated Production of Tumour Necrosis Factor-α by Monocytes.
- Authors
Debets, J. M. H.; Van Der Linden, C. J.; Spronken, I. E. M.; Buurman, W. A.
- Abstract
The aim of this study was to examine the tumour necrosis factor (TNF)-a production by peripheral blood mononuclear cells activated by mitogens. Considerable amounts of TNF-α, ranging from 1.0 to 5.0 ng/ml, were present in the supernatants of cultures of human peripheral blood mononuclear cells (PBMC), stimulated with either the anti-CD3 monoclonal antibody OKT3 or the lectin phytohaemagglutinin (PMA) The amount of TNF-α secreted in the supernatant was closely correlated to the degree of T cell proliferation in such cultures, as measured by [3H]TdR incorporation. In the absence of proliferating T cells the mitogens did not induce secretion of TNF-α by monocytes. Supernatants of proliferating T cells were shown to induce TNF-α production by monocytes. The macrophage-activating factor gamma interferon (IFN-γ) was also shown to induce, in the absence of endoloxin. TNF-α secretion by monocytes in a dose-dependent manner. The induction of TNF-α production by supernatants of proliferating T cells could largely be abrogated by passaging the supernatants on an anti- IFN-γ column before adding them to the monocytes. It is therefore concluded from this study that the production of TNF-α by monocytes can be induced by proliferating T cells and that this induction can largely be attributed to the T cell lymphokine IFN-γ.
- Subjects
T cells; TUMOR necrosis factors; BLOOD cells; MITOGENS; MONOCLONAL antibodies; LECTINS; PHYTOHEMAGGLUTININS
- Publication
Scandinavian Journal of Immunology, 1988, Vol 27, Issue 5, p601
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1988.tb02388.x