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- Title
Activation of Resting, Pure CD4<sup>+</sup>, and CD8<sup>+</sup> Cells via CD3.
- Authors
Halvorsen, R.; Gaudernack, G.; Leivestad, T.; Vartdal, F.; Thorsby, E.
- Abstract
We studied the requirements for secondary activation signals in pureCD4+ and CD8+ T cells after stimulation with anti-CD3 antibodies. Stimulation or CD4+ or CD8+ cells with anti-CD3 monoclonal antibodies (MoAb) bound to polystyrene monosized panicles never resulted in a proliferative response. However. DNA synthesis was observed when recombinant interleukin 2 (IL-2) or other secondary signals, such as those provided by phorbol myrisiate acetate (PMA) or autologous accessory cells (AC), were also added. These secondary signals were not in themselves capable of inducing DNA synthesis in the absence of particle-bound anti CD3. We also found that the signals provided by AC may be dependent on the activation state of these cells. Thus, the effects of accessory cells were enhanced by a factor present in fetal calf scrum (FCS), must likely endotoxin or lipopolysaccharide (LPS), which alone, however, were not able to activate T cells, even in the presence of particle-bound anti-CD3. Recombinant IL-1 over a broad dose range was unable to replace PMA or activated AC alter stimulation with particle-bound anti-CD3. Purified CD4+ and CD8+ T cells behaved identically in all the experiments, indicating that the basic mechanisms for activation in the two T-cell subsets are identical.
- Subjects
T cells; MONOCLONAL antibodies; DNA synthesis; PHORBOLS; INTERLEUKIN-2; ENDOTOXINS
- Publication
Scandinavian Journal of Immunology, 1987, Vol 26, Issue 2, p197
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1987.tb02252.x