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- Title
V and C Genes in Creating Antibodies Directed to α(1→3)-linked D-Glucosyls in Dextran III. Frequency of α(1→3)-Responsive, Primary B Precursors in al High- and b Low-Responder Allogroups.
- Authors
Bell, C.
- Abstract
An evaluation was made of the frequency of α(l → 3)-responsive, primary B precursors and their reactivity profiles in Igh-lal (al) and Igh-1b (b) allogroup mice, of dissimilar responsiveness as to quantity of the specific serum antibody produced, its light chain isotype, and its variable-region, ligand-modifiable antigenicity, to α(1 → 3) glucosyls in dextran B-1355. al mice, exhibiting high λ1-specific serum antibody, with relatedness to the idiotypes (Id) shared by three BALB/c, α(1 → 3)-specific, λ1 myeloma proteins, possess specific primary splenic B precursors at a frequency of 1/5.5-7.3 × 104 cells; b mice, exhibiting low, κ serum. Id- antibody, possess B splenic-specific primary precursors at a frequency of l/1.0-1.4 × 105 cells. A radioimmunoassay analysis showed correlations between the isotype profiles of the serum antibody response and of the primary precursors, namely μ λ1 in the al and μκ in the b allogroups, but also delineated minor primary μγ λ1 and μκ precursors in al and μγκ and μ λ1 in b, at frequencies of l/3 × 106 to l/6.5 × 106. The al, λ1 precursors exhibited defined reactivity patterns for 3-mono-0-3,6-di-0-glucosyls and non-reducing end groups in the B-1355. The b, κ precursors recognized the sequential residues.
- Subjects
LABORATORY mice; SERUM; IMMUNOGLOBULINS; MYELOMA proteins; IMMUNOGLOBULIN idiotypes; RADIOIMMUNOASSAY
- Publication
Scandinavian Journal of Immunology, 1982, Vol 15, Issue 1, p71
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1982.tb00623.x