We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Exorbital Lacrimal Gland Ablation and Regrafting Induce Inflammation but Not Regeneration or Dry Eye.
- Authors
Murashima, Adriana de Andrade Batista; Sant'Ana, Ariane M. S.; Faustino-Barros, Jacqueline Ferreira; Machado Filho, Elísio B.; da Silva, Lilian Costa Mendes; Fantucci, Marina Zilio; Módulo, Carolina Maria; Chahud, Fernando; Garcia, Denny Marcos; Rocha, Eduardo M.
- Abstract
The study evaluated the regenerative responses of the lacrimal functional unit (LFU) after lacrimal gland (LG) ablation. The LG of Wistar rats was submitted to G1) partial LG ablation, G2) partial ablation and transplantation of an allogeneic LG, or G3) total LG ablation, (n = 7–10/group). The eye wipe test, slit lamp image, tear flow, and histology were evaluated. RT-PCR analyzed inflammatory and proliferation mediators. The findings were compared to naïve controls after 1 and 2 months (M1 and M2). G3 presented increased corneal sensitivity, and the 3 groups showed corneal neovascularization. Histology revealed changes in the LG and corneal inflammation. In the LG, there was an increase in MMP-9 mRNA of G1 and G2 at M1 and M2, in RUNX-1 at M1 and M2 in G1, in RUNX-3 mRNA at M1 in G1, and at M2 in G2. TNF-α mRNA rose in the corneas of G1 and G2 at M2. There was an increase in the IL-1β mRNA in the trigeminal ganglion of G1 at M1. Without changes in tear flow or evidence of LG regeneration, LG ablation and grafting are unreliable models for dry eye or LG repair in rats. The surgical manipulation extended inflammation to the LFU.
- Subjects
INFLAMMATORY mediators; EYE examination; SLIT lamp microscopy; DRY eye syndromes; LABORATORY rats
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 15, p8318
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25158318