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- Title
Activation of Toll-like receptor 9 inhibits lipopolysaccharide-induced receptor activator of nuclear factor kappa- B ligand expression in rat B lymphocytes.
- Authors
Yu, Xiaoqian; Lin, Jiang; Yu, Qing; Kawai, Toshihisa; Taubman, Martin A.; Han, Xiaozhe
- Abstract
ABSTRACT B lymphocytes express multiple TLRs that regulate their cytokine production. We investigated the effect of TLR4 and TLR9 activation on receptor activator of NF-κB ligand (RANKL) expression by rat spleen B cells. Splenocytes or purified spleen B cells from Rowett rats were cultured with TLR4 ligand Escherichia coli LPS and/or TLR9 ligand CpG-oligodeoxynucleotide (CpG-ODN) for 2 days. RANKL mRNA expression and the percentage of RANKL-positive B cells were increased in rat splenocytes challenged by E. coli LPS alone. The increases were less pronounced when cells were treated with both CpG-ODN and E. coli LPS. Microarray analysis showed that expressions of multiple cyclin-dependent kinase (CDK) pathway-related genes were up-regulated only in cells treated with both E. coli LPS and CpG-ODN. This study suggests that CpG-ODN inhibits LPS-induced RANKL expression in rat B cells via regulation of the CDK pathway.
- Subjects
TOLL-like receptors; LIPOPOLYSACCHARIDES; TRANCE protein; GENE expression; B cells; CYTOKINES; LABORATORY rats; PHYSIOLOGY
- Publication
Microbiology & Immunology, 2014, Vol 58, Issue 1, p51
- ISSN
0385-5600
- Publication type
Article
- DOI
10.1111/1348-0421.12115