We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Immunomodulatory effects of Bacillus subtilis ( natto) B4 spores on murine macrophages.
- Authors
Xu, Xin; Huang, Qin; Mao, Yulong; Cui, Zhiwen; Li, Yali; Huang, Yi; Rajput, Imran Rashid; Yu, Dongyou; Li, Weifen
- Abstract
ABSTRACT To investigate the immunomodulatory effects of Bacillus subtilis ( B. subtilis) ( natto) B4 spores on murine macrophage, RAW 264.7 cells were cultured alone or with B subtilis ( natto) B4 spores at 37°C for 12 hrs, then both cells and culture supernatants were collected for analyses. Exposure of RAW 264.7 cells to B. subtilis ( natto) B4 spores had no significant effects on macrophage viability and amounts of extracellular lactate dehydrogenase (LDH). However, it remarkably increased the activities of acid phosphatase (ACP), lactate dehydrogenase (LDH) and inducible nitric oxide synthase (iNOS) in cells and the amounts of nitric oxide (NO) and cytokines (tumor necrosis factor-alpha, interferon-gamma, interleukin [IL]-1 beta, IL-6, IL-12, IL-10 and macrophage inflammatory protein-2) in culture supernatants. These results demonstrate that B. subtilis ( natto) B4 spores are harmless to murine macrophages and can stimulate their activation through up-regulation of ACP and LDH activities and enhance their immune function by increasing iNOS activity and stimulating NO and cytokine production. The above findings suggest that B. subtilis ( natto) B4 spores have immunomodulatory effects on macrophages.
- Subjects
IMMUNOMODULATORS; BACILLUS subtilis; BACTERIAL spores; IMMUNOREGULATION; MACROPHAGES; LACTATE dehydrogenase; NITRIC-oxide synthases; LABORATORY mice
- Publication
Microbiology & Immunology, 2012, Vol 56, Issue 12, p817
- ISSN
0385-5600
- Publication type
Article
- DOI
10.1111/j.1348-0421.2012.00508.x