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- Title
Listerial invasion protein internalin B promotes entry into ileal Peyer's patches in vivo.
- Authors
Chiba, Sayuri; Nagai, Takeshi; Hayashi, Toshiyuki; Baba, Yukiko; Nagai, Shigenori; Koyasu, Shigeo
- Abstract
Listeria monocytogenes (Lm) invades the host intestine using listerial invasion proteins, internalins. The in vivo role of internalin A (InlA) and internalin B (InlB) is reported here. Intragastric (i.g.) administration and ligated loop assays with Δ inlB-Lm demonstrated that a lack of InlB significantly attenuates the invasive ability of Lm into various organs. On the other hand, InlA-Lm expressing a mutant InlA with two substitutions, S192N and Y369S, which has been reported to increase the affinity of InlA to mouse E-cadherin, resulted in little increase in intestinal infection according to both ligated loop and i.g. infection assays. Lm preferentially enters ileal Peyer's patch (PP) via M cells and Δ inlB-Lm showed severely reduced ability to invade though these cells. The present results reveal the importance of InlB, which accelerates listerial invasion into M cells on ileal PPs in vivo.
- Subjects
LISTERIA monocytogenes; BACTERIAL proteins; GUT microbiome; HOSTS (Biology); GENETIC mutation
- Publication
Microbiology & Immunology, 2011, Vol 55, Issue 2, p123
- ISSN
0385-5600
- Publication type
Article
- DOI
10.1111/j.1348-0421.2010.00292.x