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- Title
Determinación del umbral de detección de Pseudococcus vïburni (Hemiptera: Pseudococcidae) por PCR.
- Authors
Vera, Diana; Garrido, Silvina; Aun, Elizabet; Lago, Jonathan; Cichon, Liliana
- Abstract
The obscure mealybug Pseudococcus viburni (Signoret) is a quarantine pest present in the Upper Valley of Río Negro and Neuquén, Argentina. The detection of any growth stage of the mealybug in quarantine inspections often results in rejections of fresh fruit in countries where the fruit is exported. In this study the detection threshold of P. viburni was determined by PCR technique. The DNA extraction kit, DNAzol™, was used as a fast DNA extraction method. By using the above extraction kit, good DNA quality and concentration was obtained from specimens of P. viburni of different developmental stages (including eggs, nymphs and adults) which were preserved in pro-analysis ethanol at -20°C following the manufacturer protocol. One aliquote of this DNA was used as a template in a PCR reaction using specific P. viburni primers recorded in the literature and DNA of P. viburni irom an entomological collection was used as a control. In all stages the amplification generated an expected size band of 650 bp visualized in an agarose 1.5% gel. The detection threshold of P. viburni using our method was an egg. These results involve a specific detection technique for P. viburni ana reliable species identification in a space of 48 h.
- Subjects
RIO Negro (Argentina); NEUQUEN (Argentina : Province); ARGENTINA; MEALYBUGS; FRUIT diseases &; pests; INSECT DNA
- Publication
Revista de la Sociedad Entomológica Argentina, 2012, Vol 71, Issue 1/2, p151
- ISSN
0373-5680
- Publication type
Article