We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The FlbA-regulated predicted transcription factor Fum21 of <italic>Aspergillus niger</italic> is involved in fumonisin production.
- Authors
Aerts, David; Hauer, Esther E.; Ohm, Robin A.; Arentshorst, Mark; Teertstra, Wieke R.; Phippen, Christopher; Ram, Arthur F. J.; Frisvad, Jens C.; Wösten, Han A. B.
- Abstract
<italic>Aspergillus niger</italic> secretes proteins throughout the colony except for the zone that forms asexual spores called conidia. Inactivation of <italic>flbA</italic> that encodes a regulator of G-protein signaling results in colonies that are unable to reproduce asexually and that secrete proteins throughout the mycelium. In addition, the Δ<italic>flbA</italic> strain shows cell lysis and has thinner cell walls. Expression analysis showed that 38 predicted transcription factor genes are differentially expressed in strain Δ<italic>flbA</italic>. Here, the most down-regulated predicted transcription factor gene, called <italic>fum21</italic>, was inactivated. Growth, conidiation, and protein secretion were not affected in strain Δ<italic>fum21</italic>. Whole genome expression analysis revealed that 63 and 11 genes were down- and up-regulated in Δ<italic>fum21</italic>, respectively, when compared to the wild-type strain. Notably, 24 genes predicted to be involved in secondary metabolism were down-regulated in Δ<italic>fum21</italic>, including 10 out of 12 genes of the fumonisin cluster. This was accompanied by absence of fumonisin production in the deletion strain and a 25% reduction in production of pyranonigrin A. Together, these results link FlbA-mediated sporulation-inhibited secretion with mycotoxin production.
- Subjects
ASPERGILLUS niger; FUMONISINS; FUNGAL spores; CONIDIA; TRANSCRIPTION factors
- Publication
Antonie van Leeuwenhoek, 2018, Vol 111, Issue 3, p311
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-017-0952-1