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- Title
Initial support for the hypothesis that PAR<sub>2</sub> is involved in the immune response to Nippostrongylus brasiliensis in mice.
- Authors
Mark Devlin; Robin Gasser; Tom Cocks
- Abstract
Abstract Protease-activated receptor 2 (PAR2) is a cell surface receptor that detects trypsin and trypsin-like enzymes. Although the precise pathophysiological roles of PAR2 are yet to be determined, the receptor has been broadly implicated in inflammation and allergy. However, no studies have investigated the possible roles of PAR2 in hosts infected by parasitic helminths. Therefore, in this preliminary investigation, we compared the infectivity of the nematode Nippostrongylus brasiliensis in mice lacking the PAR2 gene $${\left( {{\text{PAR}}^{{ - \mathord{\left/ {\vphantom { - - }} \right. \kern-\nulldelimiterspace} - }}_{2} } \right)}$$ and in their ‘background-strain’ controls (129SV). $${\text{PAR}}^{{ - \mathord{\left/ {\vphantom { - - }} \right. \kern-\nulldelimiterspace} - }}_{2} $$ mice displayed elevated fecal egg counts and decreased levels of total serum IgE, after a subcutaneous infection with 900 infective third-stage N. brasiliensis larvae compared with 129SV mice that were not susceptible to infection. In addition, in a separate study in BALB/c mice, two immunological hallmarks of parasite infection, IgE- and IL-10-expressing lymphocytes, were shown to be augmented after the coadministration of the classic antigen ovalbumin with the PAR2-activating peptide SLIGRL (single letter amino acid sequence) but not the inactive reverse peptide LRGILS. These findings provide initial support for the proposal that PAR2 is a recognition receptor for nematode-derived proteases.
- Subjects
LABORATORY mice; IMMUNE response; DIGESTIVE enzymes; IMMUNOLOGIC diseases
- Publication
Parasitology Research, 2007, Vol 101, Issue 1, p105
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-007-0467-1