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- Title
In focus in HCB.
- Authors
Taatjes, Douglas J.; Roth, Jürgen
- Abstract
Graph In this month's editorial, we will highlight two manuscripts describing (1) the use of cationic colloidal thorium dioxide particles as an electron dense marker to investigate the glycocalyx of alveolar type I and type II cells in human and mouse lung in control samples, and following treatment with heparinase and pneumolysin; and (2) a study to examine the potential feasibility and pitfalls of using biopsy samples to analyze by immunohistochemistry mismatch repair proteins in colorectal cancer biopsies and matching surgical samples. The use of electron tomography to obtain data in the I z i dimension yielded improved images displaying very finely detailed cThO SB 2 sb particle labeling of the glycocalyx, revealing hidden details including interconnections between AEII cell microvilli. They imaged both human and mouse cThO SB 2 sb -stained lung samples untreated or treated with either heparinase or the bacterial pore-forming virulence factor pneumolysin (PLY) to disrupt the glycocalyx.
- Subjects
GLYCOCALYX; BIOMARKERS; CELL membranes; THORIUM dioxide; IMMUNOSTAINING; KIDNEY glomerulus; GLYCOSAMINOGLYCANS
- Publication
Histochemistry & Cell Biology, 2023, Vol 160, Issue 2, p79
- ISSN
0948-6143
- Publication type
Article
- DOI
10.1007/s00418-023-02225-1